THE DIVISION 2 TWITTER DOWNLOAD

THE DIVISION 2 TWITTER DOWNLOAD

Download FIGВ S2, TIF file, 2 MB.Д¬ryptococcus neoformans is a pathogenic yeast that causes lethal cryptococcal meningitis in immunocompromised patients. While the data from the second daughter (top graph) show a steady increase of fluorescence along the line drawn, the data from the first daughter show diminishment of the fluorescence in the area corresponding to the mother-bud neck, suggesting the presence of discontinuous cytoplasm between the two cells. To assess separation of the cytoplasmic signal, pixel brightness along a line perpendicular to the mother-daughter axis was plotted for both daughters as shown. (C) The cell treated as described for panel B was imaged at an earlier time point when the second daughter had not developed a septum and its cytoplasm had therefore not yet separated from the mother. In this cell, both of the daughter cells possessed the septa separating them from the mother cell. (B) A multimeric cell treated as described for panel A was imaged, and individual focal planes (Z-sections) are shown to illustrate a lack of cytoplasmic connection (based on the cytoplasmic signal of GFP-Nup107). In the example shown, the AMR had constricted by 15В min of subsequent FLC treatment (white arrowhead), and a new bud had started to emerge by 135В min (white arrow) with no detachment of the first daughter cell. (A) Time-lapse microscopy was performed with strain LC4 expressing GFP-Nup107 (to visualize the nuclear envelope green) and mCherry-Myo1 (to visualize the AMR magenta) after 6В h of preincubation with FLC.

FLC does not significantly inhibit AMR assembly and constriction.

The division 2 twitter